详细记录  
题名:A prescribed Chinese herbal medicine improves glucose profile and ameliorates oxidative stress in Goto-Kakisaki rats fed with high fat diet.
作者:WU L; LI X; ZHU H; XU P; GAO X;
来源:PLoS One. 2013;8(4):e60262. doi: 10.1371/journal.pone.0060262. Epub 2013 Apr 2. [ IF= 4.09 ] ]
URL :10.1371/journal.pone.0060262
日期:20130430
摘要:Oxidative stress (OS) plays a role in hyperglycemia induced islet beta cell
dysfunction, however, studies on classic anti-oxidants didn't show positive
results in treating diabetes. We previously demonstrated that the prescribed
Chinese herbal medicine preparation "Qing Huo Yi Hao" (QHYH) improved endothelial
function in type 2 diabetic patients. QHYH protected endothelial cells from high
glucose-induced damages by scavenging superoxide anion and reducing production of
reactive oxygen species. Its active component protected C2C12 myotubes against
palmitate-induced oxidative damage and mitochondrial dysfunction. In the present
study, we investigated whether QHYH protected islet beta cell function
exacerbated by high fat diet (HFD) in hyperglycemic GK rats. 4-week-old male rats
were randomly divided into high HFD feeding group (n = 20) and chow diet feeding
group (n = 10). Each gram of HFD contained 4.8 kcal of energy, 52% of which from
fat. Rats on HFD were further divided into 2 groups given either QHYH (3 ml/Kg/d)
or saline through gastric tube. After intervention, serum glucose concentrations
were monitored; IPGTTs were performed without anesthesia on 5 fasting rats
randomly chosen from each group on week 4 and 16. Serum malondialdehyde (MDA)
concentrations and activities of serum antioxidant enzymes were measured on week
4 and 16. Islet beta cell mass and OS marker staining was done by
immunohistochemistry on week 16. QHYH prevented the exacerbation of hyperglycemia
in HFD feeding GK rats for 12 weeks. On week 16, it improved the exacerbated
glucose tolerance and prevented the further loss of islet beta cell mass induced
by HFD. QHYH markedly decreased serum MDA concentration, increased serum catalase
(CAT) and SOD activities on week 4. However, no differences of serum glucose
concentration or OS were observed on week 16. We concluded that QHYH decreased
hyperglycemia exacerbated by HFD in GK rats by improving beta cell function
partly via its antioxidant effect.

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