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题名:Activation of apocynin-sensitive NADPH oxidase (Nox2) activity in INS-1 832/13 cells under glucotoxic conditions.
作者:MOHAMMED AM; KOWLURU A;
来源:Islets. 2013 May 21;5(3). [ IF= 1.12 ] ]
URL :N U L L
日期:20130606
摘要:Several lines of recent evidence provided compelling evidence to suggest
increased generation of reactive oxygen species (ROS) as causal for mitochondrial
dysregulation and apoptosis in islet beta-cells exposed to noxious stimuli
including high glucose, lipids and proinflammatory cytokines. Studies along these
lines are also suggestive of a significant contributory role for NADPH oxidase in
the generation of ROS under the above conditions. We have recently reported a
marked increase in the expression and activation of cytosolic components of NADPH
oxidase (p47phox, Rac1) in cell culture models of glucotoxicity and in islets
from T2DM animals (Zucker Diabetic Fatty rat) and humans. In this communication,
we provide further evidence indicating significant activation of NADPH activity
(~2-fold) in INS-1 832/13 cells exposed to chronic hyperglycemic conditions (20
mM; 48 h). We also report marked attenuation of this activity, by apocynin, a
selective inhibitor of phagocyte-like NADPH oxidase (Nox2) activity. Together,
our findings implicate Nox2 as a source for ROS generation in beta-cells exposed
to glucotoxic conditions.

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