详细记录  
题名:Long-term culture and functionality of pancreatic islets monitored using microelectrode arrays.
作者:SCHONECKER S; KRAUSHAAR U; DUFER M; SAHR A; HARDTNER C; GUENTHER E; WALTHER R; LENDECKEL U; BARTHLEN W; KRIPPEIT-DREWS P; DREWS G;
来源:Integr Biol (Camb). 2014 Mar 24. [ IF= 0.00 ] ]
URL :10.1039/c3ib40261d
日期:20140408
摘要:Extracellular recording of the glucose-induced electrical activity of mouse
islets of Langerhans on microelectrode arrays (MEAs) is an innovative and
powerful tool to address beta-cell (patho-)physiology. In a dual approach we
tested whether this technique can detect concentration-dependent drug effects as
well as characterize alterations in beta-cell activity during prolonged culture.
First we established conditions that allow long-term investigation of beta-cell
function by recording electrical activity. The results provide the first
measurements of beta-cell membrane potential oscillations of individual murine
islets during long-term culture. Oscillations were recorded for up to 34 days
after islet isolation. Importantly, the glucose dependence of electrical activity
did not change over a period of one month. Thus we can follow
electrophysiological changes of individual islets induced by alterations in the
beta-cell environment over weeks. Second, we used the MEA technique to assay
beta-cell damage induced by oxidative stress and to evaluate appropriate
protection mechanisms. Oxidative stress plays a key role in the development of
type 2 diabetes mellitus (T2DM). Examination of the acute effects of H2O2 on
electrical activity showed that the oxidant reduced the electrical activity in a
concentration-dependent manner. The superoxide dismutase mimetic, tempol,
protected against the detrimental effects of H2O2. In conclusion, we demonstrated
that MEA recordings can be used to address disease-related mechanisms and
protective interventions in beta-cells. In the future, this fundamental work
should enable the monitoring of the electrical activity of islets of Langerhans
under controlled ex vivo conditions including long-term exposure to oxidative
stress, glucolipotoxicity, and other diabetes-inducing agents.

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