详细记录  
题名:A Role for SPARC in the Moderation of Human Insulin Secretion.
作者:HARRIES LW; MCCULLOCH LJ; HOLLEY JE; RAWLING TJ; WELTERS HJ; KOS K;
来源:PLoS One. 2013 Jun 28;8(6):e68253. doi: 10.1371/journal.pone.0068253. Print 2013. [ IF= 4.09 ] ]
URL :10.1371/journal.pone.0068253
日期:20130131
摘要:AIMS/HYPOTHESIS: We have previously shown the implication of the multifunctional
protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in
insulin resistance but potential effects on beta-cell function have not been
assessed. We therefore aimed to characterise the effect of SPARC on beta-cell
function and features of diabetes. METHODS: We measured SPARC expression by
qRT-PCR in human primary pancreatic islets, adipose tissue, liver and muscle. We
then examined the relation of SPARC with glucose stimulated insulin secretion
(GSIS) in primary human islets and the effect of SPARC overexpression on GSIS in
beta cell lines. RESULTS: SPARC was expressed at measurable levels in human
islets, adipose tissue, liver and skeletal muscle, and demonstrated reduced
expression in primary islets from subjects with diabetes compared with controls
(p< = 0.05). SPARC levels were positively correlated with GSIS in islets from
control donors (p< = 0.01). Overexpression of SPARC in cultured beta-cells
resulted in a 2.4-fold increase in insulin secretion in high glucose conditions
(p< = 0.01). CONCLUSIONS: Our data suggest that levels of SPARC are reduced in
islets from donors with diabetes and that it has a role in insulin secretion, an
effect which appears independent of SPARC's modulation of obesity-induced insulin
resistance in adipose tissue.

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