详细记录  
题名:Effects of biotin supplementation in the diet on insulin secretion, islet gene expression, glucose homeostasis and beta-cell proportion.
作者:LAZO DE LA VEGA-MONROY ML; LARRIETA E; GERMAN MS; BAEZ-SALDANA A; FERNANDEZ-MEJIA C;
来源:J Nutr Biochem. 2013 Jan;24(1):169-77. doi: 10.1016/j.jnutbio.2012.03.020. Epub [ IF= 0.00 ] ]
URL :10.1016/j.jnutbio.2012.03.020
日期:20130131
摘要:Besides its role as a carboxylase cofactor, biotin has a wide repertoire of
effects on gene expression, development and metabolism. Pharmacological
concentrations of biotin enhance insulin secretion and the expression of genes
and signaling pathways that favor islet function in vitro. However, the in vivo
effects of biotin supplementation on pancreatic islet function are largely
unknown. In the present study, we investigated whether in vivo biotin
supplementation in the diet has positive effects in rodent pancreatic islets.
Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet over 8
weeks postweaning and tested for glucose homeostasis, insulin secretion, islet
gene expression and pancreatic morphometry. Insulin secretion increased from the
islets of biotin-supplemented mice, together with the messenger RNA (mRNA)
expression of several transcription factors regulating insulin expression and
secretion, including forkhead box A2, pancreatic and duodenal homeobox 1 and
hepatocyte nuclear factor 4alpha. The mRNA abundance of glucokinase, Cacna1d,
acetyl-CoA carboxylase, and insulin also increased. Consistent with these
effects, glucose tolerance improved, and glucose-stimulated serum insulin levels
increased in biotin-supplemented mice, without changes in fasting glucose levels
or insulin tolerance. Biotin supplementation augmented the proportion of beta
cells by enlarging islet size and, unexpectedly, also increased the percentage of
islets with alpha cells at the islet core. mRNA expression of neural cell
adhesion molecule 1, an adhesion protein participating in the maintenance of
islet architecture, decreased in biotin-supplemented islets. These findings
provide, for the first time, insight into how biotin supplementation exerts its
effects on function and proportion of beta cells, suggesting a role for biotin in
the prevention and treatment of diabetes.

================  评  论  部  分=================

重要性:
分类:(参照 Faculty of 1000 的分类体系)


评语:

评论密码:   返回前页  [全部]