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题名:Human Umblical Cord Derived Mesenchymal Stem Cells Can Secret Insulin In Vitro and In Vivo.
作者:BOROUJENI Z; ALEYASIN A;
来源:Biotechnol Appl Biochem. 2013 May 31. doi: 10.1002/bab.1127. [ IF= 0.00 ] ]
URL :10.1002/bab.1127
日期:20130606
摘要:Diabetes mellitus is characterized by autoimmune destruction of pancreatic beta
cells, leading to decrease insulin production. Differentiation of mesenchymal
stem cells (MSCs) into insulin producing cells offers novel ways to diabetes
treatment. MSCs can be isolated from the human umbilical cord tissue and
differentiate into insulin-secreting cells. Human umbilical cord derived stem
cells (hUDSCs) were obtained after birth, selected by plastic adhesion, and
characterized by flow cytometric analysis. Human UDSCs transduced with
Non-integrated lentivirus harboring PDX1 (Non-integrated LV-PDX1) and cultured in
differentiation medium in 21 days. Pancreatic duodenum homeobox protein-1 (PDX1)
is a transcription factor in pancreatic development. Significant expressions of
PDX1, neurogenin3 (Ngn3), glucagon, glucose transporter2 (Glut2), somatostatin
were detected by quantitative RT-PCR (P < 0.05). PDX1 and insulin proteins were
shown by immunocytochemistry analysis. The insulin secretion of hUDSCs PDX1+ in
the high-glucose medium was 1.8 muU/ml. They were used for treatment of diabetic
rats and could decrease the blood glucose level from 400 mg/dL to the normal
level in 4 days. In conclusion, our results demonstrated that hUDSCs are able to
differentiate into insulin producing cells by transduction with non-integrated
LV-PDX1. This hUDSCs PDX1+ have the potential to be used as a viable resource in
cell-based gene therapy of type 1 diabetes. This article is protected by
copyright. All rights reserved.

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