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题名:Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice.
作者:RASOULI M; ALLAUDIN ZN; OMAR AR; AHMAD Z;
来源:Curr Gene Ther. 2013 May 27;13. [ IF= 0.00 ] ]
URL :N U L L
日期:20130606
摘要:Poorly controlled diabetes mellitus can result in serious complications. Gene
therapy is increasingly being considered as an alternative approach to treat
diabetes, because of its ability to induce physiological insulin secretion and it
allows patients to escape insulin injections. The properties of gut K and
L-cells, including glucose sensitivity, the ability to process insulin and a
regulated secretion pathway support their use as surrogate beta-cells. Previous
in vitro studies have provided sufficient evidence supporting the use of these
cells for gene therapy studies. Therefore, we examined the ability of K and
L-cells to produce insulin in diabetic mice. Chitosan nanoparticles were used to
transfer the insulin gene into intestinal cells via oral administration. The
efficiency of chitosan as a gene vehicle was investigated through the use of
reporter gene. Insulin mRNA and protein expression levels were measured by RT-PCR
and ELISA, respectively. Blood glucose testing revealed that this treatment
reduced glucose levels in diabetic mice. The decrease in blood glucose level in
the first week of treatment was greater in mice with K-cell specific insulin
expression compared with mice with L-cell-specific insulin expression. These
results indicate that inducing insulin secretion in K-cells conferred a quicker
response to gene therapy.

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