详细记录  
题名:Infection of human islets of langerhans with two strains of coxsackie B virus serotype 1: Assessment of virus replication, degree of cell death and induction of genes involved in the innate immunity pathway.
作者:ANAGANDULA M; RICHARDSON SJ; OBERSTE MS; SIOOFY-KHOJINE AB; HYOTY H; MORGAN NG; KORSGREN O; FRISK G;
来源:J Med Virol. 2013 Nov 19. doi: 10.1002/jmv.23835. [ IF= 0.00 ] ]
URL :10.1002/jmv.23835
日期:20131203
摘要:Type 1 diabetes mellitus is believed to be triggered, in part, by one or more
environmental factors and human enteroviruses (HEVs) are among the candidates.
Therefore, this study has examined whether two strains of HEV may differentially
affect the induction of genes involved in pathways leading to the synthesis of
islet hormones, chemokines and cytokines in isolated, highly purified, human
islets. Isolated, purified human pancreatic islets were infected with strains of
Coxsackievirus B1.Viral replication and the degree of CPE/islet dissociation were
monitored. The expression of insulin, glucagon, CXCL10, TLR3, IF1H1, CCL5, OAS-1,
IFNbeta, and DDX58 was analyzed. Both strains replicated in islets but only one
of strain caused rapid islet dissociation/CPE. Expression of the insulin gene was
reduced during infection of islets with either viral strain but the gene encoding
glucagon was unaffected. All genes analyzed which are involved in viral sensing
and the development of innate immunity were induced by Coxsackie B viruses, with
the notable exception of TLR3. There was no qualitative difference in the
expression pattern between each strain but the magnitude of the response varied
between donors. The lack of virus induced expression of TLR3, together with the
differential regulation of IF1H1, OAS1 and IFNbeta, (each of which has
polymorphic variants influence the predisposition to type 1 diabetes), that might
result in defective clearance of virus from islet cells. The reduced expression
of the insulin gene and the unaffected expression of the gene encoding glucagon
by Coxsackie B1 infection is consistent with the preferential beta-cell tropism
of the virus. J. Med. Virol. 9999: XX-XX, 2013. (c) 2013 Wiley Periodicals, Inc.

================  评  论  部  分=================

重要性:
分类:(参照 Faculty of 1000 的分类体系)


评语:

评论密码:   返回前页  [全部]