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题名:Nitrosation of uric acid induced by nitric oxide under aerobic conditions
作者:Toshinori Suzuki
来源:Nitric Oxide[IF=----], Volume 16, Issue 2, March 2007, Pages 266-273
URL :http://dx.doi.org/10.1016/j.niox.2006.10.008
日期:070415
摘要: Toshinori Suzuki, a,

aSchool of Pharmacy, Shujitsu University, 1-6-1 Nishigawara, Okayama 703-8516, Japan

Received 17 June 2006; revised 21 October 2006. Available online 10 November 2006.

Summary

Uric acid is a well-established scavenger of reactive oxygen and nitrogen species such as hydroxyl radical and peroxynitrite. However, little attention has been paid to the relationship between uric acid and nitric oxide. This paper reports the identification and characterization of a reaction product of uric acid induced by nitric oxide. When uric acid was treated with nitric oxide gas in a neutral solution under aerobic conditions, uric acid was consumed, yielding an unknown product. The product was identified as nitrosated uric acid from mass spectrometric data, although the position of the nitroso group on the molecule was not determined. The nitrosated uric acid decomposed to several compounds including uric acid with a half-life of 2.2 min at pH 7.4 and 37 C. The incubation of nitrosated uric acid with glutathione resulted in the formation of S-nitrosoglutathione. Nitrosated uric acid was also formed in the reaction with nitric oxide donors, but not with peroxynitrite. Nitrosated uric acid was detected in human serum and urine by in vitro treatment with a nitric oxide donor. In the reaction of glutathione with the nitric oxide donor, the addition of uric acid caused an increase in the yield of S-nitrosoglutathione. These results indicate that under aerobic conditions nitric oxide can convert uric acid into its nitroso derivative, which can give a nitroso group to glutathione. Uric acid may act as a vehicle of nitric oxide in humans. 4.

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