详细记录  
题名:A Comparison of NIH-Approved Human ESC Lines.
作者:Ware CB, Nelson AM, Blau CA
来源:Stem Cells 2006 Dec 24(12):2677-84
URL :http://dx.doi.org/10.1634/stemcells.2005-0452
日期:070115
摘要:Carol B. Warea, Angelique M. Nelsona, C. Anthony Blaub aDepartment of Comparative Medicine and
bDivision of Hematology, Department of Medicine, University of Washington, Seattle, Washington, USA

Correspondence: Carol B. Ware, Ph.D., University of Washington, Box 357190, Seattle, Washington 98195, USA. Telephone: 206-616-5143; Fax: 206-685-3006; e-mail: cware@u.washington.edu

Received September 16, 2005; accepted for publication July 27, 2006.
First published online in STEM CELLS EXPRESS ? August 17, 2006.


Abstract

In October 2003, the NIH established three extramural `Exploratory Centers for Human Embryonic Stem Cell Research.` Our center acquired 15 of the 22 NIH-approved cell lines. Lines were tested for: (a) freedom from mycoplasma contamination; (b) appropriate pattern of gene expression during self-renewal and differentiation; (c) ability to adapt to uniform culture conditions; (d) ability to grow at clonal densities; (e) karyotype; (f) growth efficiency; and (g) efficiency of stable transfection following electroporation. One line harbored mycoplasma. Ten lines were converted to uniform conditions. Nine lines were fully characterized. Human ESC (hESC) lines varied markedly with respect to growth efficiency as measured by the amount of time it took to plate and double (31–57 hours), cloning efficiency (0.8%–9.2%), and stable transfection rates following electroporation (0%–53% relative to a standard mouse ESC line). One hESC line had an unstable karyotype at an early passage. Modifications of the proposed Material Transfer Agreements with hESC suppliers were required to improve accessibility to hESC lines by local researchers. The NIH-approved hESC lines vary in their behavior in culture. Many hESC lines can be maintained using culture conditions less onerous than those recommended by their suppliers. Intellectual property issues pose a significant obstacle to research using NIH-approved hESC lines.



This article is selected and evaluated by Faculty of 1000 Biology, F1000 Factor 3.0
http://www.f1000biology.com/article/id/1044096


Comments

Renee Reijo Pera
University of California, San Francisco, United States of America
CELL BIOLOGY

Confirmation
Different human embryonic stem cell (hESC) lines on the NIH registry reportedly have different baseline characteristics. In this paper, the authors directly compare multiple hESC lines in terms of growth, differentiation, and genetic stability, and confirm that different lines are remarkably diverse in terms of baseline characteristics. Despite the differences found between lines, the authors were able to convert the growth of different lines to a single protocol and successfully generate reproducible data regarding characteristics.

Competing interests: None declared
Evaluated 1 Dec 2006

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