详细记录  
题名:DEVELOPMENT: Import Controls
作者:Beverly A. Purnell
来源:Science 5 January 2007 315: 18-19
URL :http://dx.doi.org/10.1126/science.315.5808.18d
日期:070115
摘要:Editors` Choice: Highlights of the recent literature


The directed and controlled differentiation of cells is of critical importance for being able to use embryonic stem cells in a clinical setting. Yasuhara et al. have shown that a switch in a nuclear transport mechanism is involved in cell fate determination. For nuclear import, a protein with a nuclear localization signal (NLS) binds to the receptor importin-, which in turn recruits importin- to mediate translocation through the nuclear pore. They find that mouse embryonic stem (ES) cells express the subtype importin-1, whereas cells that have differentiated into neurons express importin-5. Experimental manipulation confirmed that neural differentiation can be enhanced by combining the down-regulation of importin-1 with the overexpression of importin-5. Hence, the switching of importin- subtype triggers neural differentiation of ES cells. The authors propose a mechanism by which importin- subtypes function in either the undifferentiated or differentiated state by controlling the selective import of transcription factors into the nucleus--Oct3/4 for the former and Brn2 with SOX2 in the latter--which adds yet another layer of regulation for cell fate specification, this one acting via the intracellular trafficking of transcription factors. -- BAP
Nature Cell Biol. 10.1038/ncb1521 (2006).


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