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题名:N-cadherin is a useful marker for the progenitor of cardiomyocytes differentiated from mouse ES cells in serum-free condition
作者:Masahiko Honda, Akira Kurisaki, ...
来源:Biochemical and Biophysical Research Communications, Volume 351, Issue 4, 29 December 2006, Pages 877-882
URL :http://dx.doi.org/10.1016/j.bbrc.2006.10.126
日期:061130
摘要:Masahiko Hondaa, b, Akira Kurisakic, Kiyoshi Ohnumab, Hitoshi Okochid, Tatsuo S. Hamazakic, d, , and Makoto Asashimab, c, e,

aDepartment of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
bDepartment of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo 153-8902, Japan
cICORP Organ Regeneration Project, Japan Science and Technology Agency (JST), 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan dDepartment of Tissue Regeneration, Research Institute, International Medical Center of Japan, Toyama 1-21-2, Shinjuku, Tokyo, Japan
eOrgan Development Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1, higashi, Tsukuba, Ibaraki 305-8562, Japan


Abstract

Cardiomyocytes are known to differentiate spontaneously from embryonic stem (ES) cells when they formed aggregates, so called “embryoid bodies”, in the presence of serum. In this study, we explored the induction of cardiomyocytes from mouse ES cells in chemically defined serum-free medium by using a mesoderm-inducing factor, BMP4. Comparing the different inductive methods, we found a candidate cell surface marker, N-cadherin, for cardiomyocyte progenitors from ES cells. N-cadherin-positive cells highly expressed cardiogenic markers, Nkx2.5, Tbx5, and Isl1, and showed a high differentiation rate into cardiomyocyte lineage. These results indicate that N-cadherin can be a useful cell surface marker for the progenitors of cardiomyocyte differentiated from ES cells in the serum-free culture.

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