详细记录  
题名:Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells
作者:Hui Liu, Jian Lin and Krishnendu Roy
来源:Biomaterials, Volume 27, Issue 36, December 2006, Pages 5978-5989
URL :http://dx.doi.org/doi:10.1016/j.biomaterials.2006.05.053
日期:061130
摘要:Hui Liua, 1, 2, Jian Linb, 2 and Krishnendu Royc, ,

aDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA
bDepartment of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, TX 78712, USA
cThe Institute of Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA


Abstract

We have previously demonstrated that mouse embryonic stem (ES) cells differentiated on three-dimensional (3D), highly porous, tantalum-based scaffolds (Cytomatrix?) have significantly higher hematopoietic differentiation efficiency than those cultured under conventional two-dimensional (2D) tissue culture conditions. In addition, ES cell-seeded scaffolds cultured inside spinner bioreactors showed further enhancement in hematopoiesis compared to static conditions. In the present study, we evaluated how these various biomaterial-based culture conditions, e.g. 2D vs. 3D scaffolds and static vs. dynamic, influence the global gene expression profile of differentiated ES cells. We report that compared to 2D tissue culture plates, cells differentiated on porous, Cytomatrix? scaffolds possess significantly higher expression levels of extracellular matrix (ECM)-related genes, as well as genes that regulate cell growth, proliferation and differentiation. In addition, these differences in gene expression were more pronounced in 3D dynamic culture compared to 3D static culture. We report specific genes that are either uniquely expressed under each condition or are quantitatively regulated, i.e. over expressed or inhibited by a specific culture environment. We conclude that that biomaterial-based 3D cultures, especially under dynamic conditions, might favor efficient hematopoietic differentiation of ES cells by stimulating increased expression of specific ECM proteins, growth factors and cell adhesion related genes while significantly down-regulating genes that act to inhibit expression of these molecules.

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