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题名:A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5 End Formation in Drosophila
作者:Lalith S. Gunawardane, Kuniaki Saito, Kazumichi M. Nishida, Keita Miyoshi, Yoshinori Kawamura, Tomoko Nagami, Haruhiko Siomi, and Mikiko C. Siomi
来源:Science[IF=30.927] 16 March 2007 315: 1587-1590; published online 21 February 2007 (in Reports)
URL :http://dx.doi.org/10.1126/science.1140494
日期:070415
摘要: Lalith S. Gunawardane,* Kuniaki Saito,* Kazumichi M. Nishida,* Keita Miyoshi, Yoshinori Kawamura, Tomoko Nagami, Haruhiko Siomi, Mikiko C. Siomi

Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan.

* These authors contributed equally to this work.

To whom correspondence should be addressed. E-mail: siomi@genome.tokushima-u.ac.jp (H.S.); siomim@genome.tokushima-u.ac.jp (M.C.S.)

Abstract

In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AGO3. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5 ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5 terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI. 5ku 1.

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