详细记录  
题名:E74-like factor 2 regulates valosin-containing protein expression
作者:Binglin Zhang, Yasuhiko Tomita, Ying Qiu, Juxiang He, Eiichi Morii, Shinzaburo Noguchi and Katsuyuki Aozasa
来源:Biochemical and Biophysical Research Communications[IF=3], Volume 356, Issue 3, 11 May 2007, Pages 536-541
URL :http://dx.doi.org/10.1016/j.bbrc.2007.02.160
日期:070415
摘要: Binglin Zhanga, Yasuhiko Tomitaa, , , Ying Qiub, Juxiang Hea, Eiichi Moriia, Shinzaburo Noguchic and Katsuyuki Aozasaa

aDepartment of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan
bDepartment of Pathology, Medical School of Tongji University, Shanghai, China
cDepartment of Surgical Oncology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan

Received 26 February 2007. Available online 9 March 2007.

Summary

Enhanced expression of valosin-containing protein (VCP) correlates with invasion and metastasis of cancers. To clarify the transcription mechanism of VCP, human and mouse genomic sequence was compared, revealing a 260 bp DNA sequence in the 5-flanking region of VCP gene to be highly conserved between the two, in which binding motif of E74-like factor 2/new Ets-related factor (ELF2/NERF) was identified. Chromatin immunoprecipitation assay showed binding of ELF2/NERF to the 5-flanking region of VCP gene. Knock-down of ELF2/NERF by siRNA decreased expression level of VCP. Viability of cells under tumor necrosis factor-alpha treatment significantly reduced in ELF2/NERF-knock-down breast cancer cell line. Immunohistochemical analysis on clinical breast cancer specimens showed a correlation of nuclear ELF2/NERF expression with VCP expression and proliferative activity of cells shown by Ki-67 immunohistochemistry. These findings indicate that ELF2/NERF promotes VCP transcription and that ELF2/NERFCVCP pathway might be important for cell survival and proliferation under cytokine stress. 3.

================  评  论  部  分=================

重要性:
分类:(参照 Faculty of 1000 的分类体系)


评语:

评论密码:   返回前页  [全部]