详细记录  
题名:Involvement of up-regulation of PUMA in non-steroidal anti-inflammatory drug-induced apoptosis
作者:Tomoaki Ishihara, Tatsuya Hoshino, Takushi Namba, Ken-ichiro Tanaka and Tohru Mizushima
来源:Biochemical and Biophysical Research Communications[IF=3], Volume 356, Issue 3, 11 May 2007, Pages 711-717
URL :http://dx.doi.org/10.1016/j.bbrc.2007.03.034
日期:070415
摘要: Tomoaki Ishiharaa, Tatsuya Hoshinoa, Takushi Nambaa, Ken-ichiro Tanakaa and Tohru Mizushima, a,

aGraduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan

Received 5 March 2007. Available online 13 March 2007.

Summary

NSAIDs such as celecoxib induce apoptosis in cancer cells. Although this apoptotic effect is involved in the anti-tumor activity associated with such drugs, the mechanism by which this occurs is not fully understood. We report here that various NSAIDs, including celecoxib, up-regulate PUMA, a Bcl-2 family protein with potent apoptosis-inducing activity, in human gastric carcinoma cell line, accompanying the induction of apoptosis. Experiments using siRNA and an intracellular Ca2+ chelator revealed that Ca2+-dependent up-regulation of ATF4 and CHOP is involved in this up-regulation of PUMA. The siRNA for PUMA inhibited the celecoxib-induced activation and translocation of Bax, release of cytochrome c into the cytosol and induction of apoptosis, suggesting that PUMA plays an important role in celecoxib-induced mitochondrial dysfunction and the resulting apoptosis. 8.

================  评  论  部  分=================

重要性:
分类:(参照 Faculty of 1000 的分类体系)


评语:

评论密码:   返回前页  [全部]