详细记录  
题名:Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference
作者:Wee Beng Tan, Shan Jiang and Yong Zhang
来源:Biomaterials[IF=4.698], Volume 28, Issue 8, March 2007, Pages 1565-1571
URL :http://dx.doi.org/10.1016/j.biomaterials.2006.11.018
日期:070415
摘要: Wee Beng Tana, Shan Jianga and Yong Zhangb, ,

aNUS Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore 117576, Singapore

bDivision of Bioengineering, Faculty of Engineering, Blk EA-03-12, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore

Received 9 October 2006; accepted 15 November 2006. Available online 11 December 2006.

Summary

Gene silencing using short interfering RNA (siRNA) is fast becoming an attractive approach to probe gene function in mammalian cells. Although there have been some success in the delivery of siRNA using various methods, tracking their delivery and monitoring their transfection efficiency prove to be hard without a suitable tracking agent. Therefore, a challenge lies with the design of an efficient and at the same time, self-tracking, transfection agent for RNA interference. In this paper, chitosan nanoparticles (NPs) with encapsulated quantum dots (QDs) were synthesized and used to deliver HER2/neu siRNA. Using such a construct, the delivery and transfection of the siRNA can be monitored by the presence of fluorescent QDs in the chitosan NPs. Targeted delivery of HER2 siRNA to HER2-overexpressing SKBR3 breast cancer cells was shown to be specific with chitosan/QD NP surface labeled with HER2 antibody targeting the HER2 receptors on SKBR3 cells. Gene-silencing effects of the conjugated siRNA was also established using the luciferase and HER2 ELISA assays. These self-tracking siRNA delivery NPs will also aid in the monitoring of future gene silencing studies in vivo. 16.

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