详细记录  
题名:RNA interference: Implications for cancer treatment
作者:Massimo Masiero, Giorgia Nardo, Stefano Indraccolo and Elena Favaro
来源:Molecular Aspects of Medicine[IF=----], Volume 28, Issue 1, February 2007, Pages 143-166
URL :http://dx.doi.org/10.1016/j.mam.2006.12.004
日期:070415
摘要: Massimo Masieroa, 1, Giorgia Nardoa, 1, Stefano Indraccolob and Elena Favaroa, ,

aDepartment of Oncology and Surgical Sciences, Oncology Section, University of Padova, via Gattamelata, 64, 35128 Padova, Italy
bIstituto Oncologico Veneto, via Gattamelata, 64, 35128 Padova, Italy

Received 15 December 2006; accepted 18 December 2006. Available online 11 January 2007.

Summary

RNA interference (RNAi) has emerged as one of the most important discoveries of the last years in the field of molecular biology. Following clarification of this highly conserved endogenous gene silencing mechanism, RNAi has largely been exploited as a powerful tool to uncover the function of specific genes and to understand the effects of selective gene silencing in mammalian cells both in vitro and in vivo. RNAi can be induced by direct introduction of chemically synthesized siRNAs into the cell or by the use of plasmid and viral vectors encoding for siRNA allowing a more stable RNA knockdown. Potential application of this technique both as a research tool and for therapeutic purposes has led to an extensive effort to overcome some critical constraints which may limit its successful application in vivo, including off-target and non-specific effects, as well as the relatively poor stability of siRNA. This review provides a brief overview of the RNAi mechanism and of its application in preclinical animal models of cancer. 42.

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