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题名:Healthy lifestyles are associated with higher levels of perforin, granulysin and granzymes A/B-expressing cells in peripheral blood lymphocytes ? ARTICLE
作者:Qing Li, Kanehisa Morimoto, Ari Nakadai, Tianli Qu, Hiroki Matsushima, Masao Katsumata, Takako Shimizu, Hirofumi Inagaki, Yukiyo Hirata, Kimiko Hirata, et al.
来源:Preventive Medicine[IF=2.195], Volume 44, Issue 2, February 2007, Pages 117-123
URL :http://dx.doi.org/10.1016/j.ypmed.2006.08.017
日期:070415
摘要: Qing Lia, , , Kanehisa Morimotob, Ari Nakadaia, Tianli Qub, Hiroki Matsushimab, Masao Katsumataa, Takako Shimizua, Hirofumi Inagakia, Yukiyo Hirataa, Kimiko Hirataa, Tomoyuki Kawadaa, Yuquan Lub, Kunio Nakayamab and Alan M. Krenskyc

aDepartment of Hygiene and Public Health, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
bDepartment of Social and Environmental Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
cStanford University School of Medicine, Palo Alto, CA, USA

Summary

Objective
It is well documented that natural killer (NK) cells provide host defense against tumors and viruses. We previously showed that lifestyle affects human NK and LAK activities. In order to explore the underlying mechanism, we investigated the effect of lifestyle on intracellular perforin, granulysin, and granzymes A/B in peripheral blood lymphocytes (PBL).

Methods
114 healthy male subjects, aged 20C59 years, from a large company in Osaka, Japan were selected with informed consent. The subjects were divided into groups reporting good, moderate, and poor lifestyles according to their responses on a questionnaire regarding eight health practices (cigarette smoking, alcohol consumption, sleeping hours, working hours, physical exercise, eating breakfast, balanced nutrition, and mental stress). Peripheral blood was taken, and numbers of NK, T, perforin, granulysin, and granzymes A/B-expressing cells in PBL were measured by flow cytometry.

Results
Subjects with good or moderate lifestyle showed significantly higher numbers of NK, and perforin, granulysin, and granzymes A/B-expressing cells and a significantly lower number of T cells in PBL than subjects with poor lifestyle. Among the eight health practices, cigarette smoking, physical exercise, eating breakfast, and balanced nutrition significantly affect the numbers of NK, T cells, perforin, granulysin, and/or granzymes A/B-expressing cells, and alcohol consumption significantly affects the number of granzyme A-expressing cells. On the other hand, mental stress, sleeping, and working hours had no effect on those parameters.

Conclusions
Taken together, these findings indicate that poor lifestyle significantly decreases the numbers of NK, perforin, granulysin, and granzymes A/B-expressing cells in PBL.

5.

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