详细记录  
题名:The SARS-Coronavirus Membrane protein induces apoptosis through modulating the Akt survival pathway
作者:Chak-Ming Chan, Cheuk-Wing Ma, Wood-Yee Chan and Ho Yin Edwin Chan
来源:Archives of Biochemistry and Biophysics[IF=3.152], Volume 459, Issue 2, 15 March 2007, Pages 197-207
URL :http://dx.doi.org/10.1016/j.abb.2007.01.012
日期:070415
摘要: Chak-Ming Chana, b, Cheuk-Wing Maa, c, Wood-Yee Chand and Ho Yin Edwin Chana, b, c, ,

aLaboratory of Drosophila Research, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China
bDepartment of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China
cMolecular Biotechnology Programme, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China
dDepartment of Anatomy, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China

Received 11 October 2006; revised 20 December 2006. Available online 31 January 2007.

Summary

A number of viral gene products are capable of triggering apoptotic cell death through interfering with cellular signaling cascades, including the Akt kinase pathway. In this study, the pro-apoptotic role of the SARS-CoV Membrane (M) structural protein is described. We found that the SARS-CoV M protein induced apoptosis in both HEK293T cells and transgenic Drosophila. We further showed that M protein-induced apoptosis involved mitochondrial release of cytochrome c protein, and could be suppressed by caspase inhibitors. Over-expression of M caused a dominant rough-eye phenotype in adult Drosophila. By performing a forward genetic modifier screen, we identified phosphoinositide-dependent kinase-1 (PDK-1) as a dominant suppressor of M-induced apoptotic cell death. Both PDK-1 and Akt kinases play essential roles in the cell survival signaling pathway. Altogether, our data show that SARS-CoV M protein induces apoptosis through the modulation of the cellular Akt pro-survival pathway and mitochondrial cytochrome c release. 20.

================  评  论  部  分=================

重要性:
分类:(参照 Faculty of 1000 的分类体系)


评语:

评论密码:   返回前页  [全部]