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ISBN: 3527308261
题名: Handbook of Rna Biochemistry
作者: Hartmann
出版社: John Wiley & Sons
页数: 968p
目录: This practice-oriented handbook covers all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. It has been written with the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex and is based on the latest research in RNA methodology. RNA SYNTHESIS Enyzmatic synthesis, ligation and modification Chemical synthesis STRUCTURE DETERMINATION Sequence determination Secondary and tertiary structure determination Footprinting techniques Identification of interacting proteins and nucleic acids Nucleotide analog interference mapping Atomic structure determination Spectroscopic studies of RNA structure GENOMICS & BIOINFORMATICS Phylogenetic analysis Structure prediction Molecular dynamics simulation RNA mining and RNomics ANALYSIS OF RNA FUNCTION Isolation and purification of RNA-binding proteins Genetic analysis of RNA-binding proteins Three-hybrid analysis to identify RNA-binding proteins Analysis of mRNA splice variants Confocal microscopy studies of intracellular localization In vitro selection of ribozymes and aptamers RNA INTERFERENCE Strategies for in vivo RNAi studies (partial contents)
编目日期: 2005-6-2 0:00:00
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