|文摘: ||This book has the ambition to provide the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many chapters of this book deal with the purification, the detection and the characterization of the different forms of this protein. In addition, in vitro, cellular and animal models specifically adapted to the study of TSEs, as well as bio-safety procedures are described. Each chapter is covered by scientists involved for many years in their respective domain of prion biology and who give the best of their knowledge in this technical document. This volume is a very useful tool for any laboratory which recently decided to contribute to the study of TSEs as well as for teams already engaged in this field for many years but interested in extending their technical capacity toward other unknown methods.
Preface.- Abbreviations.- Transmissible Spongiform Encephalopathies or Prion Diseases (General Introduction).- Purification of PrPC.- Purification of the Pathological Isoform of Prion Protein (PrPsc or PrP-res) from Transmissible Spongiform Encephalopathy-affected Brain Tissue.- Production and Purification of Recombinant PrP.- Animal Models of TSEs.- Cell Culture Models of TSEs.- PrPsc Immunohistochemistry.- Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies.- Antibody Production and ELISA, Diagnosis of TSEs, General Considerations.- Strain Typing in Mice.- Molecular Strain Typing.- Biosafety and Decontamination Procedures.- Cell-free Conversion of Prion Proteins.- Cytotoxicity of PrP Peptides.- Cyclic Amplification of Prion Protein Misfolding.- Guide to Solutions.- Guide to Protocols.- Index.